Fractionation


  1. After you've done the lab:
    1. Follow up information and questions about the lab:
      1. Figures 1-5 are micrographs of chloroplasts that were isolated from spinach (Spinacea oleracea) in a student lab last week.
      2. Measure the dimensions of the chloroplasts, referring to the 10m m bar shown in Figure 6. If you calibrate a ruler by referring to Figure 6, you can make precise measurements!

 

 

Fig. 1. Chloroplast - untreated (control) - note thylakoids

Fig. 2. Chloroplast - treated with saturated urea (approx. 30 min). Note blebbing of membrane.

Fig. 3. Chloroplast - treated with saturated urea (2 hours)

 

Fig. 4. Chloroplast - treated with saturated urea (20 hours)

 

Fig. 5. Chloroplast - treated with saturated urea (20 hours)

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Fig. 6. The white line above represents 10 m m

 

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