BIOGRAPHICAL SKETCH

Kenneth W. Olsen Phone: 312-508-3121

Professor of Chemistry FAX: 312-508-3086

Loyola University of Chicago e-mail: kolsen@luc.edu

6525 N. Sheridan Road

Chicago, IL 60626

EDUCATION:

Institution & Location Degree Year Major

Iowa State University, Ames, Iowa B. S. 1967 Biochemistry

Duke University, Durham, NC Ph. D. 1972 Biochemistry

Purdue University, W. Lafayette, IN Post-Doc. 1975 Protein Crystallography

PROFESSIONAL EXPERIENCE:

Position Employer Date

Research Assistant D. J. Graves 9/64 - 8/67

Iowa State Univ.

Research Associate M. G. Rossmann 5/72 - 8/75

Purdue Univ.

Assistant Professor A. J. Wahba 8/75 - 8/83

of Biochemistry U. Miss. Med. Ctr.

Associate Professor S. F. Pavkovic 8/83 - 7/91

of Chemistry Loyola Univ.

Professor of Chemistry S. F. Pavkovic 8/91 - present

Loyola University

Visiting Scientist M. Karplus 8/92 - 12/92

Harvard University

Chair of Chemistry Loyola University 8/93 - present

HONORS:

Phi Eta Sigma(1964), Pi Mu Epsilon(1965), Phi Lamda Upsilon (1966), Phi Kappa Phi (1966), Sigma Xi (1976), NSF Undergrad. Research Fellowship (1964-67), PHS Trainee (1967-72), American Cancer Soc. Postdoct. Fellow (1972-75), American Society of Biological Chemists Travel Award (1976), Member of Special NIH Study Sect. on Biological Instrumentation (1986), Argonne Summer Research Fellow (1986), American Society for Biochemistry and Molecular Biology Travel Award (1988), Fellowship Review Committee of the American Heart Association of Metropolitan Chicago (1988, 1990), Ad Hoc Member of NIH Biomedical Sciences Study Section (1992, 1993, 1994), NIH HLBP Program Project Review Committee (1995, 1998), NIH Bioengineering Project Review Committee (1999)

MEMBERSHIPS:

American Crystallographic Association, American Chemical Society, American Society for Biochemistry and Molecular Biology, Biophysical Society, Protein Society

PUBLICATIONS:

Trayer, I.P. and Olsen, K.W.:  Affinity-Chromatography Studies on the A Protein of Lactose Synthetase.  Biochem. J. 127:9-10, 1972.

Barker, R., Olsen, K.W., Shaper, J.H. and Hill, R.L.:  Agarose Derivatives of Uridine Diphosphate and N-Acetylglucosamine for the Purification of a Galactosyltransferase.  J. Biol. Chem. 247:  7135-7147, 1972.

Hill, R.L., Barker, R., Olsen, K.W., Shaper, J.H. and Trayer, I.P.: Lactose Synthetase: Structure and Function.  In "Metabolic Interconversion of Enzymes" (Wieland, O., Ed.), Springer-Verlag, Berlin, pp. 331-346, 1972.

Buehner, M., Ford, G.C., Moras, D., Olsen, K.W. and Rossmann, M.G.:D-Glyceraldehyde-3-Phosphate Dehydrogenase:  Three-Dimensional Structure and Evolutionary Significance.  Proc. Natl. Acad. Sci.U.S.A. 70:3052-3054, 1973.

Buehner, M., Ford, G.C., Moras, D., Olsen, K.W. and Rossmann, M.G.:Structure Determination of Crystalline Lobster D-Glyceraldehyde-3-Phosphate Dehydrogenase.  J. Mol.  Biol. 82:563-585, 1974.

Eventoff, W., Olsen, K.W. and Hackert, M.L.:  The Purification of Porcine Heart Lactate Dehydrogenase by Affinity Chromatography.  Biochim. Biophys. Acta 341:327-331, 1974.

Rossmann, M.G., Moras, D. and Olsen, K.W.:  Chemical and Biological Evolution of Nucleotide Binding Protein.  Nature 250:194-199, 1974.

Buehner, M., Ford, G.C., Moras, D., Olsen, K.W. and Rossmann, M.G.: Three-Dimensional Structure of D-Glyceraldehyde3-Phosphate Dehydrogenase.  J. Mol. Biol. 90:25-49, 1974.

Brew, K., Shaper, J.H., Olsen, K.W., Trayer, I.P. and Hill, R.L.:Crosslinking of the Components of Lactose Synthetase with Diemethylpimelimidate.  J. Biol. Chem. 250:1434-1444, 1975.

Olsen, K.W.:  Lysozyme - a book review.  Amer. Scientist 63:469, 1975.

Moras, D., Olsen, K.W., Sabesan, M.N., Buehner, M., Ford, G.C. and Rossmann, M.G.:  Asymmetry in the Three-Dimensional Structure of Lobster Holo D-Glyceraldehyde-3-Phosphate Dehydrogenase. J.Biol. Chem. 250: 9137-9162, 1975.

Olsen, K.W., Moras, D., Rossmann, M.G. and Harris, J.I.:  Sequence Variability and the Structure of D-Glyceraldehyde-3-Phosphate Dehydrogenase.  J. Biol. Chem.  250:9313-9321, 1975.

Olsen, K.W., Garavito, R.M., Sabesan, M.N. and Rossmann, M.G.: Anion Binding Sites in the Active Center of D-Glyceraldehyde-3-Phosphate Dehydrogenase.  J. Mol. Biol.  107:571-576, 1976.

Olsen, K.W., Garavito, R.m., Sabesan, M.N. and Rossmann, M.G.: Studies on Coenzyme Binding to Glyceraldehyde-3-Phosphate Dehydrogenase.  J. Mol. Biol. 107:577-584, 1976.

Olsen, K.W.:  Affinity Chromatography.  Amer. Lab. 9:21-28, 1977.

Olsen, K.W.:  Three-Dimensional Structure of Hemoglobin: CRC Handbook Series in Clinical Laboratory Science Section I. Hematology, edited by R. M. Schmidt. CRC Press, pp. 105-177, 1979.

Wells, R.L. and Olsen, K.W.:  Comparison of Temperature Effect on the Guanidine Hydrochloride and Urea Denaturations of Lysozyme.  Physiol. Chem. Phys. 11:345-351, 1979.

Olsen, K.W.:  Internal Residue Criteria for Predicting Three-Dimensional Protein Structures.  Biochim. Biophys. Acta 622: 259-267, 1980.

Olsen, K.W.:  A New Method for Affinity Chromatography of Heme Binding Proteins:  Synthesis and Characterization of Hematin- and Hematoporphyrin-agarose.  Analyt. Biochem. 109:250-254, 1980.

Woodley, C.L., Roychowdhury, M., MacRae, T.H., Olsen, K.W. and Wahba, A.J.:  Protein Synthesis in Brine Shrimp Embryos II.  Regulation of Ternary Complex (Met-tRNA*eIf-2*GTP) Formation by Two Purified Protein Factors and Phosphorylation of Artemia eIF-2.  Eur. J. Biochem. 117: 543-551, 1981.

Moo-Penn, W.F., McPhedran, P., Bobrow, S., Johnson, M.H., Jue, D.L.and Olsen, K.W.:Hemoglobin Connecticut (β21(B3) AspGly): A Hemoglobin Variant with Low Oxygen Affinity.  Am. J. Hemat.11:137-145, 1981.

Olsen, K.W. and Miller, R.L.:  Crystallization and Preliminary X-ray Data for Peanut Agglutinin.  FEBS Lett. 145:303-307, 1982.

Olsen, K.W.:  Using PROPHET to Predict Abnormal Hemoglobin Structures.  In "Scientific Applications of the PROPHET System" (H.M. Perry, ed.) Bolt Beranek and Newman, Inc., Cambridge, Mass., pp. 17-1 to 17-9, 1982.

Olsen, K.W.:  Prediction of Three-Dimensional Structure of Plant Lectins from the Domains of Concanavalin A.  Biochim. Biophys. Acta 743: 212-218, 1983.

Olsen, K.W.:  Structural Basis for the Thermal Stability of Glyceraldehyde-3-Phosphate Dehydrogenases.  Int. J.  Peptide Protein Res. 22: 469-475, 1983.

Olsen, K.W.:  Prediction of the Three-Dimensional Structures of Plant Lectins.  In "Chemical Taxonomy, Molecular Biology, and Function of Plant Lectins" (M. Etzler and I. Goldstein, eds.) Alan R. Liss, Inc., New York, pp. 261-264, 1983.

Olsen, K.W.:  Three-Dimensional Structure of Hemoglobin.  In "CRC Handbook Series in Clinical Laboratory Science, Section I: Hematology, Volume IV" (R.M. Schmidt and V.F. Fairbanks, ed.) CRC Press, Boca Raton, FL, pp. 45 - 124, 1986.

Olsen, K.W.:  Affinity Chromatography of Heme Binding Proteins: Synthesis and Characterization of Hematin- and Hematoporphyrin- Agarose, Methods in Enzymol.  123:324-331, 1986.

White, F.L. and Olsen, K.W.:  Effects of Cross-Linking on the Thermal Stability of Hemoglobin I.  The Use of Bis (3,5-Dibromosalicyl) Fumarate, Arch. Biochem. Biophys. 258:51-57, 1987.

Yang, T. and Olsen, K.W.: Effect of Crosslinking on the Thermal Stability of Hemoglobins II: The Stabilization of Met, Cyanomet and Carbonmonoxy Hemoglobins A and S with Bis(3,5-Dibromosalicyl) Fumarate, Arch. Biochem. Biophys. 261:283-290, 1988.

Moo-Penn, W.F., Jue, D.L., Johnson, M.H., Olsen, K.W., Shih, T.-B., Jones, R.T., Lux, S.E. and Arnone, A.: Hemoglobin Brockton [β138 (H16) Ala Pro]: An Unstable Variant with Normal Oxygen Affinity, Biochemistry 27:7614-7619, 1988.

Castignetti, D., Siddiqui, A.F. and Olsen, K.W.: A Spectrophotometric Assay for the Degradation of the Siderophore Deferrioxamine B, J. Biochem. Biophys. Methods 17:119-126, 1988.

Yang, T. and Olsen, K.W.: Thermal Stability and Crosslinking of Hb New York [β113(G15) ValGlu)], Hemoglobin 13:147-156, 1989.

Schiffer, M., Ainsworth, C., Xu, Z.-B., Carperos, W., Olsen, K., Solomon, A., Stevens, F.J., and Chang, C.-H.: Structure of a Second Crystal Form of Bence-Jones Protein Loc: Strikingly Different Domain Associations in Two Crystal Forms of a Single Protein, Biochemistry 28:4066-4072, 1989.

Yang, T. and Olsen, K.W.: The Effect of Crosslinking by Bis(3,5-Dibromosalicyl) Fumarate on the Autooxidation of Hemoglobin, Biochem. Biophys. Res. Commun. 163:733-738, 1989.

Zaluzec, E.J., Ekabo, O., Yung, M., Pavkovic, S.F., and Olsen, K.W.: Binding and Structural Studies on Peanut Agglutinin, In " Lectins: Biology, Biochemistry, Clinical Biochemistry" (J. Kocourek and D. L. J. Freed, eds.), Sigma Chemical Co., St. Louis, pp. 151-160, 1990.

Yang, T. and Olsen, K.W.: Thermal Stability of Hemoglobin O Indonesia (α116 GluLys), Hemoglobin, 14:641-646, 1990.

Yang, T. and Olsen, K.W.: Thermal Stability of Hemoglobin Crosslinked in the T-State by Bis(3,5-Dibromosalicyl) Fumarate, Biochem. Biophys. Res. Commun. 174:518-523, 1991.

Zaluzec, E.J., Zaluzec, M.M., Olsen, K.W., and Pavkovic, S.F.: Crystallization and Preliminary X-Ray Analysis of Peanut Agglutinin/N6-Benzylaminopurine Complex, J. Mol. Biol., 219:151-153, 1991.

Olsen, K.W., Zhang, Q.-Y., Huang H., Sabaliauskas, G.K., and Yang, T.: Stabilities and Properties of Multilinked Hemoglobins, Biomater., Artif. Cells, and Immob. Biotech., 20:283-286, 1992.

Olsen, K.W.: Thermal Denaturation Procedures for Hemoglobin, Methods Enzymol. 231:514-524, 1994.

Huang, H. and Olsen, K.W.: Thermal Stabilities of Hemoglobins Crosslinked with Different Length Reagents, Biomater., Artif. Cells, and Immob. Biotech., 22:719-724, 1994.

Yang, T. and Olsen, K.W.: Enzymatic Protection from Autoxidation for Crosslinked Hemoglobins, Biomater., Artif. Cells, and Immob. Biotech., 22:709-717, 1994.

Zhang, Q. and Olsen, K.W.: The Modification of Hemoglobin by a Long Crosslinking Reagent: Bis(3,5-Dibromosalicyl) Sebacate, Biochem. Biophys. Res. Commun., 203:1463-1470, 1994.

Pavlik, P. A., Boyd, M. K. and Olsen, K. W.: Molecular Dynamics of a Hemoglobin Crosslinking Reaction, Biopolymers, 39:615-618, 1996.

Zheng, Y. and Olsen, K. W.: Tris(3,5-Dibromosalicyl) Tricarballylate Crosslinked Hemoglobin: Functional Evaluation, Artif. Cells, Blood Subs. and Immob. Biotech., 24: 587-598, 1996.

Lin, W., Zhang, Q., Olsen, K. W. and Mota de Freitas, D., ANMR and O2 Affinity Study of Cs+ Binding in Human Erythrocytes: Implications for Cs+ Toxicity,@ Arch. Biochem. Biophys., 369: 78-88, 1999.

Fernandez, E. J., Abad-Zapatero, C. and Olsen, K. W., ACrystal Structure of Lysβ182- Lysβ282 Crosslinked Hemoglobin: A Possible Allosteric Intermediate,@ J. Mol. Biol., 296:1245-1256, 2000.