Research Interests

The lab's primary focus is to investigate and develop novel approaches to reduce complications of open fractures. These complications include delayed bone healing, non-unions, recalcitrant infections, and deranged inflammation. The lab is also interested in elucidating immune mechanisms underlying delayed healing of fractures following major musculoskeletal trauma including polytrauma such as those sustained in military and civilian populations. Immune paralysis occurs following major traumatic injuries (blunt trauma, burn, fractures, hemorrhage, etc.) and is believed to contribute to delayed/impaired wound healing. One arm of my research interests focuses on investigating the underlying cellular signaling pathways responsible for creating immune paralysis after major trauma (polytrauma), with a goal to identify inflammatory mediators that will severe as potential therapeutic targets to alleviate immune paralysis and potentiate timely wound healing. Key words Osteoimmunology Fracture related infections Trauma immunology Inflammation Wound healing

Publications

• ME Whitely, AL Lofgren, SM Shiels, JC Wenke Fracture healing in a polytrauma rat model is influenced by mtDNA:cGAS complex mediated pro-inflammation JOURNAL OF EXPERIMENTAL ORTHOPAEDICS ; 10 1 90
• PJ Muire, AL Lofgren, SM Shiels, JC Wenke Fracture healing in a Polytrauma Rat Model Is Influenced by mtDNA:cGAS Complex Mediated Pro-Inflammation (in press) JOURNAL OF EXPERIMENTAL ORTHOPAEDICS
• PJ Muire, MA Thompson, RJ Christy, S Natesan Advances in immunomodulation and immune engineering approaches to improve healing of extremity wounds International journal of molecular sciences ; 23 8 4074
• ME Whitely, SM Helms, PJ Muire, AL Lofgren, RA Lopez, JC Wenke Preclinical evaluation of a commercially available biofilm disrupting wound lavage for musculoskeletal trauma JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH ; 17 1 347
• PJ Muire, JJ Avila, AL Lofgren, JC Wenke Neutralization of HMGB1 improves fracture healing and ?d T lymphocyte counts at the fracture site in a polytrauma rat model JOURNAL OF EXPERIMENTAL ORTHOPAEDICS ; 9 1 1-12
• SM Shiels, PJ Muire, JC Wenke FK506 increases susceptibility to musculoskeletal infection in a rodent model BMC MUSCULOSKELETAL DISORDERS ; 23 1 1-7
• VG Gude, PJ Muire Preparing for outbreaks–implications for resilient water utility operations and services SUSTAINABLE CITIES AND SOCIETY ; 64 64 102558
• PJ Muire, MG Schwacha, JC Wenke Systemic T cell exhaustion dynamics is linked to early high mobility group box protein 1 (HMGB1) driven hyper-inflammation in a polytrauma rat model Cells ; 10 7 1646
• PJ Muire, LH Mangum, LC Mangum, GR Garcia, DR Romano, JC Wenke Characterization of the local and systemic temporal changes to the lipid mediator and cytokine cascades following osteotomy and polytrauma Journal of Immunology ; 204 1_Supplement 144.3
• PJ Muire, LH Mangum, JC Wenke Time course of immune response and immunomodulation during normal and delayed healing of musculoskeletal wounds Frontiers in immunology ; 11 1056
• A Krishnavajhala, PJ Muire, L Hanson, H Wan, F McCarthy, A Zhou, L Petrie-Hanson Transcriptome Changes Associated with Protective Immunity in T and B Cell-Deficient Rag1-/-Mutant Zebrafish INTERNATIONAL JOURNAL OF IMMUNOLOGY ; 5 2 20-36
• PJ Muire, LA Hanson, R Wills, L Petrie-Hanson Differential gene expression following TLR stimulation in rag1-/- mutant zebrafish tissues and morphological descriptions of lymphocyte-like cell populations PLoS ONE ; 12 9 e0184077
• PJ Muire, L Hanson, J Yoder, L Petrie-Hanson Transcript analysis of natural killer (NK) cell specific genes in the liver, kidney and spleen tissues of rag1-/- mutant zebrafish in response to in vivo administration of TLR ligands THE JOURNAL OF IMMUNOLOGY ; 196 216.4
• PJ Muire, L Hanson, L Petrie-Hanson Differential gene expression following stimulation of TLRs 2, 3, 7 and 8 in rag1-/-mutant zebrafish THE JOURNAL OF IMMUNOLOGY ; 194 1_Supplement 57.3
• PJ Muire, LH Mangum, JJ Avila, AL Lofgren, JC Wenke Single dose of anti-high mobility group box 1 protein (HMGB-1) neutralizing antibody ameliorates dysregulated inflammation and restores fracture healing in a polytrauma rat model Journal of Immunology ; 204 1_Supplement 144.7