Loyola University Chicago

Department of Biology

Imaging Lab Equipment

Transmission Electron Microscopy

LKB 7801 Knifemaker (2)

Sorvall GKM Knifemaker (for EM use)

Sorvall MT-1 Microtome (2)

Sorvall MT-2B Ultramicrotome

Sorvall MT-5000 Ultramicrotome (2)

LKB Ultratome Nova

JEOL JEE-4X Vacuum Evaporator

JEOL 1200 EX Transmission EM - film recorder, transmitted beam only, NO detectors


Scanning Electron Microscopy

Polaron Critical Point Dryer (3 basket chamber)

Hummer VI Sputter coater - Gold/Palladium source

Hummer 6.2 Sputtering System - Gold/Palladium source

SPI module sputter coater - Gold/Palladium source

Cambridge Instrument S240 Scanning EM - Kodak D90 digital capture system


Light Microscopy Preparation

AO Spenser 860 Sliding Microtome (2)

BioRad Microcut H1200 Vibratome

AO Spenser 820 Rotary microtome (at least 2)

Sorvall JB-4 microtome

Sorvall Glass Knike Maker (for light microscope histology use)

EMS Oscillating Tissue Slicer

Sorvall TC-2 Tissue Sectioner


Digital Imaging - Microscopes

Olympus BH2 compound scope - 4x, 10x, 40x, 100x oil objectives with Bright-field and DIC

40x and 100x oil phase objectives

UV burner with - BF, U, B and G cubes (slide only holds 3... G normally not in place)

Q-Imaging camera

Sony Hyper HAD CCD-IRIS RGB camera hooked to a Panasonic Time Lapse VCR

Olympus SZX-12 stereomicroscope

0.5x and 2.0x lenses on revolving nose piece

SZX-ILLD 100 light base

Drawing tube

Q-Imaging camera

both microscopes attached to a PC with Image Pro Premier 9.0 and Image Pro Discovery as the Image

capture software


Fluorescence Microscopy

Nikon Labophot compound microscope with 10x, 40x, 100x objectives, Bright-field & Dark-field

            10x and 20x phase objectives

            UV burner with B and Chroma ‘TRITC’ cubes

            Nikon D-5000 imaging system

Nikon Optiphot compound microscope with 10x, 40x, 100x objectives, Bright-field & Dark-field

            10x and 20x phase objectives

            UV burner with B and G cubes

            Nikon D-5000 imaging system

Zeiss Axiovert 100TV inverted microscope, 10x and 20x objectives

(not sure what the cubes are in this microscope)

SPOT imaging system with attached monochrome-camera


Confocal Microscopy
Olympus Fluoview FV1000 Laser Scanning Confocal system

Olympus BX61WI fixed stage upright microscope, 4x, 10x, 20x, 60x, 60x oil, 100x oil objectives

extra 4x, 20x water, and 60x water objectives available

located on a Vibraplane air table

Lasers - HeNe (633), DPSS (561), Diode (440), and Argon Ion (488 and 514)

separate work station for reviewing data sets

Olympus DSU Confocal system

            Olympus IX81 inverted microscope, 10x, 20x, 60x oil, 100x objectives

            Tokai Hit stage top incubation system

            Andor Neo camera

            Metamorph Imaging software


Photography / Documentation

Bencher copy stand system

digital workstation with Photoshop CS3, and attached Epson Perfection 4990 Photo scanner

heated drying cabinet

laser printers - Dell 2330dn b/w, HP Color Laserjet CP2025… both networked

Seal Compress 110S & Commercial 210M dry mounting presses

digital, 35mm, 120mm, & video cameras available

darkroom inside BIF

1 gal autorad developing tank (manual)

Durst Laborator 138 S b/w enlarger - 35mm thru 4"x5" negative size, point source bulb

Polaroid MP3 copy system using Fuji 100B style film packs... UV transilluminator light box

5 gal autorad developing tank (manual)


Sample preparation

Generally, most researchers do sample prep in own labs, however a shared prep room is available with…

two 5' hoods; various ovens for infiltration and embedding of samples; assorted standard bench tools - pH meter, digital balance, osmometer; Barnstead E-Pure water system; house system - compressed air, gas, vacuum, distilled water and internet.