Flow Cytometry Core Facility

Phong Le, PhD, Core Facility Director
ple@luc.edu

Bert Ladd, Core Facilities Manager
rladd@luc.edu
CBCC Room 212

Lauren Martin, Flow Cytometry Specialist
lmartin18@luc.edu

The Flow Cytometry Core Facility is in Room 212 of the Cardinal Bernardin Cancer Center. Investigators, students, and staff can be trained to operate the analyzers independently, or request staff-operated analyzer and cell sorter appointments by emailing rladd@luc.edu. Consultation and analysis available for an additional charge.

Basic consultation on experiment/panel design is available free-of-charge.
In-depth design work will incur the hourly Consultation/Analysis charge at the discretion of core staff.

PC Workstations with analysis software (FlowJo, FCAP Array, etc.) are available free-of-charge, but time must be reserved on the portal.
Basic consultation on analysis is available free-of-charge.
In-depth analysis or analysis consultation will incur the hourly Consultation/Analysis charge at the discretion of core staff.

Data acquired in the facility is uploaded to the Shared Drive (\\fshsd01\V03\ONChsd\Shared\FACS) the following business day. Email rladd@luc.edu to request access.
Data acquired in the facility is backed up to a physical archive for redundancy.
DISCLAIMER: The Shared Drive and physical archive are not intended to be used as primary data storage. Download your data and back it up to a separate location ASAP.

Users can request staff-operated acquisition of their samples. Must be approved by staff in advance of booking.
Users can request cell sorting for isolation of cell populations. Must be approved by staff in advance, as this service is entirely staff-operated.

Users can be trained to independently acquire data on all instruments except the cell sorters (AriaIIIu and Aurora CS), which is entirely staff-operated.
- LSR Fortessa (BD) 4-Laser Conventional Flow Cytometer
- Novocyte Quanteon (Agilent) 4-Laser Conventional Flow Cytometer
- Aurora (Cytek) 5-Laser Full-Spectrum Flow Cytometer
- Amnis ImageStream MkII (Cytek) 3-Laser Imaging Flow Cytometer
Users can be trained on basic data analysis via FlowJo; consultation available for advanced analysis.
An introductory lecture for new users of the facility is offered roughly every 6 weeks.

$75 for 1st hour, then $38 per hour.

Basic: free-of-charge
In-Depth: $60 per hour

Independent: $10 per 30 minutes, minimum charge $16
Staff-Operated: $75 per hour

$150 flat fee
- Includes introductory lecture, hands-on training, and basic analysis training.

4 Lasers: 405nm, 488nm, 640nm, 785nm
6 channels for imaging: fluorescence and/or brightfield
Imaging data output allows analysis of co-localization, internalization, etc.

5 Lasers: 355nm, 405nm, 488nm, 561nm, 633nm
64 channels for fluorescence, up to ~40 fluorescent parameters simultaneously
Automated loader for 96-well plates and 40-tube rack

5 Lasers: 355nm, 405nm, 488nm, 561nm, 633nm
64 channels for fluorescence, up to ~40 fluorescent parameters simultaneously
Droplet-based isolation of up to 6 simultaneous cell populations based on scatter and fluorescence

Single-cell barcoding for downstream RNA sequencing

4 Lasers: 405nm, 488nm, 561nm, 633nm
15 channels for fluorescent parameters
Droplet-based isolation of up to 4 cell populations based on scatter and fluorescence

4 Lasers: 405nm, 488nm, 561nm, 633nm
15 channels for fluorescent parameters

4 Lasers: 405nm, 488nm, 561nm, 633nm
20 channels for fluorescent parameters
Photodetectors with high dynamic range – minimal voltage adjustment required
Automated loader for plates (96-well etc.) and 40-tube rack

Phong Le, PhD, Core Facility Director
ple@luc.edu

Bert Ladd, Core Facilities Manager
rladd@luc.edu
CBCC Room 212

Lauren Martin, Flow Cytometry Specialist
lmartin18@luc.edu

Flow Cytometry Core Facility

Services

Consultation

Data Analysis

Data Management

Staff-Operated Acquisition and Cell Sorting

Training/Education

Pricing

Cell Sorting (staff operated only)

Consultation/Analysis

Data Acquisition

Training for independent data acquisition

Instruments

Amnis ImageStream MkII (Cytek) 3-Laser Imaging Flow Cytometer

Aurora (Cytek) 5-Laser Full-Spectrum Flow Cytometer

Aurora CS (Cytek) 5-Laser Full-Spectrum Cell Sorter

Chromium X Controller (10X Genomics)

FACS AriaIIIu (BD) 4-Laser Conventional Cell Sorter

LSR Fortessa (BD) 4-Laser Conventional Flow Cytometer

Novocyte Quanteon (Agilent) 4-Laser Conventional Flow Cytometer